FLIPR Tetra high-throughput real-time fluorescence detection and analysis system

Equipped with an automatic sampling system with multiple fluxes (96/384/1536 channels), using LED as the light source and a high-sensitivity CCD camera to achieve synchronous sampling and real-time detection of the entire board signal (both fluorescence and chemiluminescence can be detected), accurately reproducing the rapid dynamic response process of the receptor or ion channel. Suitable for basic research in life sciences and high-throughput drug screening.


Product Overview

FLIPRDetra is a high-throughput CCD fluorescence detection and analysis system, suitable for life science research and high-throughput drug screening, such as molecular biology, botany, genetics, zoology, microbiology, pathology, and pathophysiology. Especially in the calcium flow detection of GPCRs in drug screening, it can quantitatively detect intracellular calcium flow. Calcium flow detection is an important detection indicator in biology. Internationally, the quantitative detection technology for calcium flow in GPCRs is becoming increasingly widely used as a compound screening technique. FLIPR can also be used for more accurate fluorescence quantitative qualitative detection, using CCD direct imaging, which is more suitable for the increasing number of GPCR cytology studies. And FLIPRTetra has a highly sensitive chemiluminescence detection system, which uses ICCD imaging to perform high-sensitivity reporter gene analysis such as luciferase. FLIPR utilizes various techniques to provide reliable analysis data from multiple methods and angles, taking into account both accuracy and functionality.

main features

FLIPR is an instrument that integrates a liquid addition system and a detection system. It is a combination of a workstation and a CCD imaging reader. FLIPR can be equipped with a 96/384/1536 liquid addition system and a fast calcium flow fluorescence detection system. The entire plate can be filled with liquid simultaneously, and CCD whole plate imaging can be performed with an imaging interval of 0.2-60s and high-quality data, fully meeting our experimental requirements.
2. FLIPR can be equipped with LED and filter combinations for fluorescence detection and chemiluminescence detection at different wavelengths. We also develop matching test kits for different detection indicators to ensure high-quality results. Simultaneously, it can seamlessly integrate with the robotic arm, ensuring the integrity of experimental design and achieving maximum automation of experimental operations. The optional sampling head, LED, and filter are very easy to replace, without the need for any tools, and can be operated by laboratory personnel. EMCCD can also be upgraded to ICCD to meet the detection requirements of fluorescence and chemiluminescence.
To meet the specificity of cell experiments, FLIPR has also specially designed a cell culture pool. An external liquid circulation cleaning system can be used, and the cell suspension can be directly placed in the cell culture pool. Simply edit the template in the software and click the mouse to perform cell sampling. Meanwhile, this area can also be used for cleaning the gun head during the experimental process.
4. The screenwork software that comes with FLIPR is powerful and easy to use. Real time detection of fluorescence/chemiluminescence results can be obtained, and IC50 results can also be fitted in one step.

application area

Mainly used for life science research and high-throughput drug screening, such as molecular biology, botany, genetics, zoology, microbiology, pathology, and pathophysiology. It mainly includes several major aspects:
(1) Real time measurement of intracellular calcium signal changes for monitoring GPCR receptor activity;
(2) Real time measurement of cell membrane potential changes for monitoring ion channel activity;
(3) Early toxicity evaluation of myocardial safety;
(4) Real time monitoring of intracellular pH changes.